{"id":7746,"date":"2024-02-15T18:26:13","date_gmt":"2024-02-15T17:26:13","guid":{"rendered":"https:\/\/genetika-plzen.nullable.group\/?page_id=7746"},"modified":"2024-02-15T18:27:48","modified_gmt":"2024-02-15T17:27:48","slug":"fish-fluorescencni-in-situ-hybridizace","status":"publish","type":"page","link":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/","title":{"rendered":"FISH: Fluorescent In Situ Hybridization"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"7746\" class=\"elementor elementor-7746\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f2637e3 e-flex e-con-boxed e-con e-parent\" data-id=\"f2637e3\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-52706e0 elementor-widget elementor-widget-spacer\" data-id=\"52706e0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1ec661f e-flex e-con-boxed e-con e-parent\" data-id=\"1ec661f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3626b3e elementor-widget elementor-widget-text-editor\" data-id=\"3626b3e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>FISH is a microscopic molecular-cytogenetic method that allows targeted examination of predetermined chromosomal regions\/genes. In our laboratory, the FISH method is currently used for preimplantation genetic screening (PGS (PGT-A)) of selected chromosomes (usually the 5 most common whole-chromosome numerical abnormalities) and for preimplantation genetic diagnosis\/testing of unbalanced forms of familial rearrangements (PGT-SR) of small extent, where the NGS method cannot be used.<\/p>\n\n<p><strong>Principle of the FISH Method:<\/strong> The examination is conducted on cell nuclei fixed on a microscope slide. The principle of the method involves labeling several specific chromosomal regions with a fluorescently labeled probe complementary to the given DNA segment. Evaluation is performed using fluorescence microscopy. The method can only determine numerical changes in selected chromosomes or their parts, marked by the chosen fluorescent probe. When performing PGD (PGT-SR) to detect unbalanced forms of familial chromosomal rearrangement, it involves parts of chromosomes participating in this aberration. PGD (PGT-SR) by the FISH method is usually preceded by chromosomal examination of the parents to verify the accuracy of the proposed combination of fluorescent probes (so-called \"set-up\").<\/p>\n\n<p><strong>Limitations of the FISH Method:&nbsp;<\/strong>FISH cannot exclude any other diseases or developmental defects of the fetus that are not caused by changes in the number of examined chromosomal regions.<\/p>\n\n<h3>Schema of FISH Examination \u2013 PGT-A, PGT-SR<\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c5c898c e-flex e-con-boxed e-con e-parent\" data-id=\"c5c898c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ed3fe6c elementor-widget elementor-widget-image\" data-id=\"ed3fe6c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"539\" height=\"188\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/schema_FISH.jpg\" class=\"attachment-large size-large wp-image-7748\" alt=\"\" srcset=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/schema_FISH.jpg 539w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/schema_FISH-300x105.jpg 300w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/schema_FISH-18x6.jpg 18w\" sizes=\"(max-width: 539px) 100vw, 539px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-041ed9e e-flex e-con-boxed e-con e-parent\" data-id=\"041ed9e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e211bb1 elementor-widget elementor-widget-text-editor\" data-id=\"e211bb1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>Example of PGT-SR results using the FISH method<\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-69efefe e-flex e-con-boxed e-con e-parent\" data-id=\"69efefe\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-548e844 elementor-widget elementor-widget-image\" data-id=\"548e844\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"518\" height=\"250\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/FISH_priklad_vysledku.jpg\" class=\"attachment-large size-large wp-image-7749\" alt=\"\" srcset=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/FISH_priklad_vysledku.jpg 518w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/FISH_priklad_vysledku-300x145.jpg 300w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/FISH_priklad_vysledku-18x9.jpg 18w\" sizes=\"(max-width: 518px) 100vw, 518px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-87a0c74 e-flex e-con-boxed e-con e-parent\" data-id=\"87a0c74\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-bd231bd elementor-widget elementor-widget-image-gallery\" data-id=\"bd231bd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image-gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-image-gallery\">\n\t\t\t<div id='gallery-1' class='gallery galleryid-7746 gallery-columns-4 gallery-size-thumbnail'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"bd231bd\" data-elementor-lightbox-title=\"fish_2\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6Nzc1MCwidXJsIjoiaHR0cHM6XC9cL2dlbmV0aWthLXBsemVuLm51bGxhYmxlLmdyb3VwXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wMlwvZmlzaF8yLmpwZyIsInNsaWRlc2hvdyI6ImJkMjMxYmQifQ%3D%3D\" href='https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2.jpg'><img decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" srcset=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2-150x150.jpg 150w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2-300x300.jpg 300w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2-12x12.jpg 12w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_2.jpg 670w\" sizes=\"(max-width: 150px) 100vw, 150px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"bd231bd\" data-elementor-lightbox-title=\"fish_1\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6Nzc1MSwidXJsIjoiaHR0cHM6XC9cL2dlbmV0aWthLXBsemVuLm51bGxhYmxlLmdyb3VwXC93cC1jb250ZW50XC91cGxvYWRzXC8yMDI0XC8wMlwvZmlzaF8xLmpwZyIsInNsaWRlc2hvdyI6ImJkMjMxYmQifQ%3D%3D\" href='https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_1.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/fish_1-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-8ba3d90 e-flex e-con-boxed e-con e-parent\" data-id=\"8ba3d90\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-25ece0c elementor-widget elementor-widget-spacer\" data-id=\"25ece0c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>FISH je mikroskopick\u00e1 molekul\u00e1rn\u011b-cytogenetick\u00e1 metoda umo\u017e\u0148uj\u00edc\u00ed c\u00edlen\u00e9 vy\u0161et\u0159en\u00ed p\u0159edem ur\u010den\u00fdch chromosomov\u00fdch oblast\u00ed\/gen\u016f. Vy\u0161et\u0159en\u00ed metodou FISH je v&nbsp;sou\u010dasnosti v&nbsp;na\u0161\u00ed laborato\u0159i pou\u017e\u00edv\u00e1no pro&nbsp;preimplanta\u010dn\u00ed genetick\u00fd screening (PGS&nbsp;(PGT-A)) vybran\u00fdch chromosom\u016f (obvykle 5&nbsp;nej\u010dast\u011bj\u0161\u00edch celochromosomov\u00fdch po\u010detn\u00edch odchylek) a&nbsp;pro&nbsp;preimplanta\u010dn\u00ed genetickou diagnostiku\/testov\u00e1n\u00ed nebalancovan\u00fdch forem famili\u00e1rn\u00edch p\u0159estaveb (PGT-SR) mal\u00e9ho rozsahu, kde nelze pou\u017e\u00edt metodu NGS. Princip metody FISH: Vy\u0161et\u0159ov\u00e1na jsou bun\u011b\u010dn\u00e1 j\u00e1dra fixovan\u00e1 na&nbsp;podlo\u017en\u00ed [&hellip;]<\/p>","protected":false},"author":9,"featured_media":0,"parent":7368,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"service-area":[32],"class_list":["post-7746","page","type-page","status-publish","hentry","service-area-vysetreni-reprodukcni-genetiky"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace | Genetika Plze\u0148<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace | Genetika Plze\u0148\" \/>\n<meta property=\"og:description\" content=\"FISH je mikroskopick\u00e1 molekul\u00e1rn\u011b-cytogenetick\u00e1 metoda umo\u017e\u0148uj\u00edc\u00ed c\u00edlen\u00e9 vy\u0161et\u0159en\u00ed p\u0159edem ur\u010den\u00fdch chromosomov\u00fdch oblast\u00ed\/gen\u016f. Vy\u0161et\u0159en\u00ed metodou FISH je v&nbsp;sou\u010dasnosti v&nbsp;na\u0161\u00ed laborato\u0159i pou\u017e\u00edv\u00e1no pro&nbsp;preimplanta\u010dn\u00ed genetick\u00fd screening (PGS&nbsp;(PGT-A)) vybran\u00fdch chromosom\u016f (obvykle 5&nbsp;nej\u010dast\u011bj\u0161\u00edch celochromosomov\u00fdch po\u010detn\u00edch odchylek) a&nbsp;pro&nbsp;preimplanta\u010dn\u00ed genetickou diagnostiku\/testov\u00e1n\u00ed nebalancovan\u00fdch forem famili\u00e1rn\u00edch p\u0159estaveb (PGT-SR) mal\u00e9ho rozsahu, kde nelze pou\u017e\u00edt metodu NGS. Princip metody FISH: Vy\u0161et\u0159ov\u00e1na jsou bun\u011b\u010dn\u00e1 j\u00e1dra fixovan\u00e1 na&nbsp;podlo\u017en\u00ed [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/\" \/>\n<meta property=\"og:site_name\" content=\"Genetika Plze\u0148\" \/>\n<meta property=\"article:modified_time\" content=\"2024-02-15T17:27:48+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/schema_FISH.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/\",\"name\":\"FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace | Genetika Plze\u0148\",\"isPartOf\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#website\"},\"datePublished\":\"2024-02-15T17:26:13+00:00\",\"dateModified\":\"2024-02-15T17:27:48+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/genetika-plzen.nullable.group\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Vy\u0161et\u0159en\u00ed reproduk\u010dn\u00ed genetiky\",\"item\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#website\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/\",\"name\":\"Genetika Plze\u0148\",\"description\":\"Genetika Plze\u0148\",\"publisher\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/genetika-plzen.nullable.group\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#organization\",\"name\":\"Genetika Plze\u0148\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg\",\"contentUrl\":\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg\",\"width\":286,\"height\":95,\"caption\":\"Genetika Plze\u0148\"},\"image\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace | Genetika Plze\u0148","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/fish-fluorescencni-in-situ-hybridizace\/","og_locale":"en_GB","og_type":"article","og_title":"FISH: Fluorescen\u010dn\u00ed In Situ Hybridizace | Genetika Plze\u0148","og_description":"FISH je mikroskopick\u00e1 molekul\u00e1rn\u011b-cytogenetick\u00e1 metoda umo\u017e\u0148uj\u00edc\u00ed c\u00edlen\u00e9 vy\u0161et\u0159en\u00ed p\u0159edem ur\u010den\u00fdch chromosomov\u00fdch oblast\u00ed\/gen\u016f. Vy\u0161et\u0159en\u00ed metodou FISH je v&nbsp;sou\u010dasnosti v&nbsp;na\u0161\u00ed laborato\u0159i pou\u017e\u00edv\u00e1no pro&nbsp;preimplanta\u010dn\u00ed genetick\u00fd screening (PGS&nbsp;(PGT-A)) vybran\u00fdch chromosom\u016f (obvykle 5&nbsp;nej\u010dast\u011bj\u0161\u00edch celochromosomov\u00fdch po\u010detn\u00edch odchylek) a&nbsp;pro&nbsp;preimplanta\u010dn\u00ed genetickou diagnostiku\/testov\u00e1n\u00ed nebalancovan\u00fdch forem famili\u00e1rn\u00edch p\u0159estaveb (PGT-SR) mal\u00e9ho rozsahu, kde nelze pou\u017e\u00edt metodu NGS. 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