{"id":7826,"date":"2024-02-15T18:44:49","date_gmt":"2024-02-15T17:44:49","guid":{"rendered":"https:\/\/genetika-plzen.nullable.group\/?page_id=7826"},"modified":"2024-02-15T18:45:43","modified_gmt":"2024-02-15T17:45:43","slug":"karyomapping","status":"publish","type":"page","link":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/","title":{"rendered":"Karyomapping"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"7826\" class=\"elementor elementor-7826\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7dcd409 e-flex e-con-boxed e-con e-parent\" data-id=\"7dcd409\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ca2497d elementor-widget elementor-widget-spacer\" data-id=\"ca2497d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-efdade8 e-flex e-con-boxed e-con e-parent\" data-id=\"efdade8\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0645333 elementor-widget elementor-widget-text-editor\" data-id=\"0645333\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Karyomapping is a robust modern molecular-genetic chip examination of variants in the DNA sequence. The uniqueness of the set of these variants in each individual forms the basis of so-called \"indirect\" genetic diagnostics, which is fundamental for PGT-M testing. It is a whole-genome examination, and in addition to testing any monogenic disease, it also allows a preliminary screening examination of quantitative changes in all chromosomes (PGT-A).<\/p>\n\n<p><strong>Principle of the Karyomapping Method:<\/strong> The method is based on comparing the DNA of embryonic samples with the DNA of the couple and a reference family member, collected during the SET-UP. DNA is extracted from the collected samples, and a special whole genome amplification (WGA of the MDA type) is additionally performed on embryonic samples. The examination itself is performed on a special type of microchip (SNParray), which can simultaneously detect a large number of selected single nucleotide polymorphisms (SNPs) of the entire genome. The specific combination of SNPs in the gene region causing the given monogenic disease distinguishes individual individuals (family members, embryos) and both their alleles of this gene (haplotypes). By comparing the identified haplotypes with pedigree information of the examined family, haplotypes associated with the presence of the mutation (i.e., carrying the risk for the disease) are determined. In some cases, which occur very rarely, it is necessary to use Karyomapping in combination with direct mutation detection. The method is universal and allows the examination of any known monogenic disease. Another advantage is the significant reduction in complexity and shortening of the preparatory phase of the examination (SET-UP).<\/p>\n\n<p><strong>Limitations of the Karyomapping Method:<\/strong> PGD for familial monogenic diseases (PGT-M) cannot exclude any other diseases or developmental defects of the fetus that are not caused by a mutation in the given gene. Karyomapping cannot detect de novo mutations in embryonic DNA. <em>de novo<\/em> v&nbsp;embryon\u00e1ln\u00ed DNA.<\/p>\n\n<p>Karyomapping, as a method primarily intended for excluding monogenic diseases, does not guarantee the detection of random aneuploidies at a level comparable to aCGH or NGS. Aneuploidy screening by Karyomapping is only an additional examination that can increase the success of treatment by selecting the most promising embryo, and it is offered at no extra charge.<\/p>\n\n<h3>Schema of Karyomapping Examination \u2013 PGT-M<\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-79e3b5d e-flex e-con-boxed e-con e-parent\" data-id=\"79e3b5d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0bce6d9 elementor-widget elementor-widget-image\" data-id=\"0bce6d9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"515\" height=\"310\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema.jpg\" class=\"attachment-large size-large wp-image-7829\" alt=\"\" srcset=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema.jpg 515w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema-300x181.jpg 300w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema-18x12.jpg 18w\" sizes=\"(max-width: 515px) 100vw, 515px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b11330a elementor-widget elementor-widget-image\" data-id=\"b11330a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"447\" height=\"277\" src=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/princip_vysetreni_kryo.png\" class=\"attachment-large size-large wp-image-7830\" alt=\"\" srcset=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/princip_vysetreni_kryo.png 447w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/princip_vysetreni_kryo-300x186.png 300w, https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/princip_vysetreni_kryo-18x12.png 18w\" sizes=\"(max-width: 447px) 100vw, 447px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-581c4ff e-flex e-con-boxed e-con e-parent\" data-id=\"581c4ff\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1128347 elementor-widget elementor-widget-spacer\" data-id=\"1128347\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Karyomapping je robustn\u00ed modern\u00ed molekul\u00e1rn\u011b-genetick\u00e9 \u010dipov\u00e9 vy\u0161et\u0159en\u00ed variant v&nbsp;sekvenci DNA.&nbsp;Na&nbsp;unicit\u011b souboru t\u011bchto variant u&nbsp;ka\u017ed\u00e9ho jedince je zalo\u017eena tzv. \u201enep\u0159\u00edm\u00e1\u201c genetick\u00e1 diagnostika, kter\u00e1 je z\u00e1kladem pro&nbsp;vy\u0161et\u0159en\u00ed PGT-M.&nbsp;Jedn\u00e1 se&nbsp;o&nbsp;vy\u0161et\u0159en\u00ed cel\u00e9ho genomu, krom\u011b vy\u0161et\u0159en\u00ed jak\u00e9hokoli monogenn\u00edho onemocn\u011bn\u00ed tedy z\u00e1rove\u0148 umo\u017e\u0148uje orienta\u010dn\u00ed screeningov\u00e9 vy\u0161et\u0159en\u00ed kvantitativn\u00edch zm\u011bn v\u0161ech chromosom\u016f (PGT-A). Princip metody Karyomapping: Metoda je zalo\u017eena na&nbsp;porovn\u00e1n\u00ed DNA&nbsp;embryon\u00e1ln\u00edch vzork\u016f a&nbsp;DNA&nbsp;p\u00e1ru [&hellip;]<\/p>","protected":false},"author":9,"featured_media":0,"parent":7368,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"service-area":[32],"class_list":["post-7826","page","type-page","status-publish","hentry","service-area-vysetreni-reprodukcni-genetiky"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Karyomapping | Genetika Plze\u0148<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Karyomapping | Genetika Plze\u0148\" \/>\n<meta property=\"og:description\" content=\"Karyomapping je robustn\u00ed modern\u00ed molekul\u00e1rn\u011b-genetick\u00e9 \u010dipov\u00e9 vy\u0161et\u0159en\u00ed variant v&nbsp;sekvenci DNA.&nbsp;Na&nbsp;unicit\u011b souboru t\u011bchto variant u&nbsp;ka\u017ed\u00e9ho jedince je zalo\u017eena tzv. \u201enep\u0159\u00edm\u00e1\u201c genetick\u00e1 diagnostika, kter\u00e1 je z\u00e1kladem pro&nbsp;vy\u0161et\u0159en\u00ed PGT-M.&nbsp;Jedn\u00e1 se&nbsp;o&nbsp;vy\u0161et\u0159en\u00ed cel\u00e9ho genomu, krom\u011b vy\u0161et\u0159en\u00ed jak\u00e9hokoli monogenn\u00edho onemocn\u011bn\u00ed tedy z\u00e1rove\u0148 umo\u017e\u0148uje orienta\u010dn\u00ed screeningov\u00e9 vy\u0161et\u0159en\u00ed kvantitativn\u00edch zm\u011bn v\u0161ech chromosom\u016f (PGT-A). Princip metody Karyomapping: Metoda je zalo\u017eena na&nbsp;porovn\u00e1n\u00ed DNA&nbsp;embryon\u00e1ln\u00edch vzork\u016f a&nbsp;DNA&nbsp;p\u00e1ru [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/\" \/>\n<meta property=\"og:site_name\" content=\"Genetika Plze\u0148\" \/>\n<meta property=\"article:modified_time\" content=\"2024-02-15T17:45:43+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/\",\"name\":\"Karyomapping | Genetika Plze\u0148\",\"isPartOf\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#website\"},\"datePublished\":\"2024-02-15T17:44:49+00:00\",\"dateModified\":\"2024-02-15T17:45:43+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/genetika-plzen.nullable.group\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Vy\u0161et\u0159en\u00ed reproduk\u010dn\u00ed genetiky\",\"item\":\"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Karyomapping\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#website\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/\",\"name\":\"Genetika Plze\u0148\",\"description\":\"Genetika Plze\u0148\",\"publisher\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/genetika-plzen.nullable.group\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#organization\",\"name\":\"Genetika Plze\u0148\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg\",\"contentUrl\":\"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg\",\"width\":286,\"height\":95,\"caption\":\"Genetika Plze\u0148\"},\"image\":{\"@id\":\"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Karyomapping | Genetika Plze\u0148","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/","og_locale":"en_GB","og_type":"article","og_title":"Karyomapping | Genetika Plze\u0148","og_description":"Karyomapping je robustn\u00ed modern\u00ed molekul\u00e1rn\u011b-genetick\u00e9 \u010dipov\u00e9 vy\u0161et\u0159en\u00ed variant v&nbsp;sekvenci DNA.&nbsp;Na&nbsp;unicit\u011b souboru t\u011bchto variant u&nbsp;ka\u017ed\u00e9ho jedince je zalo\u017eena tzv. \u201enep\u0159\u00edm\u00e1\u201c genetick\u00e1 diagnostika, kter\u00e1 je z\u00e1kladem pro&nbsp;vy\u0161et\u0159en\u00ed PGT-M.&nbsp;Jedn\u00e1 se&nbsp;o&nbsp;vy\u0161et\u0159en\u00ed cel\u00e9ho genomu, krom\u011b vy\u0161et\u0159en\u00ed jak\u00e9hokoli monogenn\u00edho onemocn\u011bn\u00ed tedy z\u00e1rove\u0148 umo\u017e\u0148uje orienta\u010dn\u00ed screeningov\u00e9 vy\u0161et\u0159en\u00ed kvantitativn\u00edch zm\u011bn v\u0161ech chromosom\u016f (PGT-A). Princip metody Karyomapping: Metoda je zalo\u017eena na&nbsp;porovn\u00e1n\u00ed DNA&nbsp;embryon\u00e1ln\u00edch vzork\u016f a&nbsp;DNA&nbsp;p\u00e1ru [&hellip;]","og_url":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/","og_site_name":"Genetika Plze\u0148","article_modified_time":"2024-02-15T17:45:43+00:00","og_image":[{"url":"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2024\/02\/kryomapping_schema.jpg"}],"twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/","url":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/","name":"Karyomapping | Genetika Plze\u0148","isPartOf":{"@id":"https:\/\/genetika-plzen.nullable.group\/#website"},"datePublished":"2024-02-15T17:44:49+00:00","dateModified":"2024-02-15T17:45:43+00:00","breadcrumb":{"@id":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/genetika-plzen.nullable.group\/en\/vysetreni-reprodukcni-genetiky\/karyomapping\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/genetika-plzen.nullable.group\/"},{"@type":"ListItem","position":2,"name":"Vy\u0161et\u0159en\u00ed reproduk\u010dn\u00ed genetiky","item":"https:\/\/genetika-plzen.nullable.group\/vysetreni-reprodukcni-genetiky\/"},{"@type":"ListItem","position":3,"name":"Karyomapping"}]},{"@type":"WebSite","@id":"https:\/\/genetika-plzen.nullable.group\/#website","url":"https:\/\/genetika-plzen.nullable.group\/","name":"Genetika Plze\u0148","description":"Genetika Plze\u0148","publisher":{"@id":"https:\/\/genetika-plzen.nullable.group\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/genetika-plzen.nullable.group\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/genetika-plzen.nullable.group\/#organization","name":"Genetika Plze\u0148","url":"https:\/\/genetika-plzen.nullable.group\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/","url":"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg","contentUrl":"https:\/\/genetika-plzen.nullable.group\/wp-content\/uploads\/2022\/07\/logo2.svg","width":286,"height":95,"caption":"Genetika Plze\u0148"},"image":{"@id":"https:\/\/genetika-plzen.nullable.group\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/pages\/7826","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/comments?post=7826"}],"version-history":[{"count":4,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/pages\/7826\/revisions"}],"predecessor-version":[{"id":7833,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/pages\/7826\/revisions\/7833"}],"up":[{"embeddable":true,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/pages\/7368"}],"wp:attachment":[{"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/media?parent=7826"}],"wp:term":[{"taxonomy":"service-area","embeddable":true,"href":"https:\/\/genetika-plzen.nullable.group\/en\/wp-json\/wp\/v2\/service-area?post=7826"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}